280LAMBS BORN AFTER IN VITRO EMBRYO PRODUCTION FROM PREPUBERTAL LAMB OOCYTES AND FROZEN-THAWED UNSORTED AND SEX-SORTED SPERMATOZOA
نویسندگان
چکیده
منابع مشابه
Developmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa
The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...
متن کاملDevelopmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa
The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...
متن کاملIn vitro characterisation of fresh and frozen sex-sorted bull spermatozoa.
This study sought to compare the in vitro characteristics of fresh and frozen non-sorted (NS) and sex-sorted (SS) bull spermatozoa. Experiment 1: Holstein-Friesian ejaculates (n=10 bulls) were split across four treatments and processed: (1) NS fresh at 3×106 spermatozoa, (2) X-SS frozen at 2×106 spermatozoa, (3) X-SS fresh at 2×106 spermatozoa and (4) X-SS fresh at 1×106 spermatozoa. NS frozen ...
متن کاملIn vitro production of bovine embryos using flow-cytometrically sexed sperm
The investigation aimed to compare the effect of fresh and frozen-thawed X and Y fractions of flowcytometrically sorted bovine spermatozoa on in vitro fertilization of bovine in vitro matured oocytes and subsequent blastocyst development. Sperm cells sorted in MoFloSX cytometer were used either for IVF or frozen and stored in liquid nitrogen. Immature oocytes recovered from ovaries of slaughter...
متن کاملFrozen-thawed embryo transfer cycles
In order to reduce the chance of multiple pregnancy during in-vitro fertilisation (IVF)– embryo transfer treatment, there is an increasing trend towards transferring a lower number of embryos. Surplus good-quality embryos are then transferred in frozen-thawed embryo transfer (FET) cycles. The first pregnancy after FET was reported in 1983.1 It has been estimated that in IVF programmes in which ...
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ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2004
ISSN: 1031-3613
DOI: 10.1071/rdv16n1ab280